Determination of CTLA-4 levels in spleen tissue of rats treated with ginger oil (Zingiber Officinale) by immunohistochemical methods

Abstract

This study was investigated to determine CTLA-4 levels in the spleen tissue of rats treated with ginger oil (Zingiber officinale) by immunohistochemical methods. For the study, 21 male Sprague-dawley rats of 250-300 g weight were used. The groups were formed as control group, ginger 100 mg/kg group and ginger 500 mg/kg group. Histological and immunohistochemical methods were applied to the spleen tissues taken at the end of the study. Red and white pulp, central artery, trabeculae and vena trabecularis were found to have normal histological structure in the spleen tissue of all groups. Weak CTLA-4 immunoreactivity was detected in the red and white pulp in the spleen tissue of the control and ginger 100 mg/kg groups, and moderate CTLA-4 immunoreactivity was detected in the ginger 500 mg/kg group. In our study, it was determined that ginger oil administration at different doses did not cause histological changes in the spleen tissue and increased CTLA-4 immunoreactivity in the ginger 500 mg/kg group. Based on our results, it is concluded that in order to fully explain the effects of ginger administration on the spleen, it is necessary to determine the efficacy of different doses, forms and administration forms of ginger.