Therapeutic effect of pomegranate peel extract on heme oxygen-free 1 (HO-1) and angiotensin-converting enzyme-2 (ACE-2) in the kidney tissue of mice treated with mitomycin
Effect of pomegranate peel on HO-1 and ACE-2 in the kidney tissue of mice treated with mitomycin
DOI:
https://doi.org/10.5281/zenodo.10444360Keywords:
Kidney, heme oxygenase-1, angiotensin-converting enzyme-2, pomegranate peel, mitomycin-CAbstract
This study aimed to investigate the effects of pomegranate peel (PP) extract on the damage caused by mitomycin-C (MMC) in kidney tissue. In the study, 32 female Mus musculus albino mice were divided into 4 groups, as; Control Group: distilled water was administered via oral gavage for 15 days. MMC Group: distilled water was administered via oral gavage for 15 days. On day 15, MMC was administered at a dose of 2 mg/kg i.p. MMC+150PP Group: 150 mg/kg of PP extract was administered via oral gavage for 15 days. On day 15, MMC was administered at a dose of 2 mg/kg i.p. MMC+300PP Group: 300 mg/kg of PP extract was administered via oral gavage for 15 days. On day 15, MMC was administered at a dose of 2 mg/kg i.p. Crossman's triple staining method was employed to examine the histological structure of the kidney tissue. Additionally, the Streptavidin-Biotin Peroxidase complex technique was applied to determine the immunoreactivity of heme oxygenase-1 (HO-1) and angiotensin-converting enzyme-2 (ACE-2). The application of MMC caused degeneration in the kidney tissue, but when MMC was used in combination with PP extract, it significantly reduced the existing degeneration in the kidney tissue. HO-1 immunoreactivity was similar in the control and MMC+300PP groups, but it was higher in the MMC+150PP group compared to the control and MMC+300PP groups, although it was lower than in the MMC group. ACE-2 immunoreactivity was weak in the control and MMC+300PP groups, moderate in the MMC+150PP group, but intense in the MMC group. ACE-2 and HO-1 immunoreactivity were not observed in the medulla and vascular endothelium of the kidney tissues in any of the groups. It was determined that MMC causes damage to kidney tissue, and it was found that PP extract reduces this damage. Based on these findings, it is believed that PP extract can be used in the prevention and treatment of kidney diseases.
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