Effects of hydrogen-enriched water on lipid profile and some biochemical markers: Effects of hydrogen-enriched water on lipid profile

Mahmut Karapehlivan; Ömer Faruk Başer; Turgut Dolanbay; Duried Alwazeer

doi:10.5281/zenodo.10444706

Authors

Mahmut Karapehlivan Kafkas University Medicine School, Department of Medical Biochemistry, Kars, Türkiye https://orcid.org/0000-0003-0408-534X
Ömer Faruk Başer Kafkas University Medicine School, Department of Medical Biochemistry, Kars, Türkiye https://orcid.org/0000-0002-7201-4490
Turgut Dolanbay Niğde Ömer Halisdemir University Medicine School, Department of Emergency Medicine, Niğde, Türkiye https://orcid.org/0000-0002-4092-1192
Duried Alwazeer Iğdır University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Iğdır, Türkiye https://orcid.org/0000-0002-2291-1628

DOI:

https://doi.org/10.5281/zenodo.10444706

Keywords:

Molecular hydrogen, antioxidant, biochemical parameter

Abstract

Molecular hydrogen (H2) is a non-toxic, tasteless, colorless, and odorless gas with high diffusion properties in living tissues and cells . By scavenging dangerous free radicals like the hydroxyl radical (•OH) and peroxynitrite (ONOO-) within cells, H2 functions as a selective antioxidant. It was aimed to determine the usability of molecular hydrogen (H2) as a new 'pro-metabolic' agent that may affect liver health. The study was initiated with the permission of 2023-154 obtained from Kafkas University Animal Experiments Local Ethics Committee. A total of 14 4-month-old male Wistar Albino rats weighing 200-250 grams, with seven rats in each group, were used in the study. Rats were fed ad-libitium throughout the study. The rats were housed in a 12-hour light and 12-hour dark environment at appropriate temperature. The Unpaired t-test was analyzed for each biochemical parameter to determine the difference between the two groups. A significant P-value was defined as less than 0.05. GraphPad 9.5.1 (San Diego, CA, USA) was used for statistical analysis. Results showed that AST, ALT, total protein, total cholesterol, total cholesterol, and LDL were significantly lower in the H2 group compared to the control group (P<0.05). There was no statistically significant difference between the group given H2 and the control group between HDL and TG (P>0.05). In conclusion, We think that H2 suppresses oxidative stress, reduces TG, TC, and LDL, which are responsible for vascular occlusion, and can be used for treatment purposes for the liver and many systems and tissues in the future.

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Effects of hydrogen-enriched water on lipid profile and some biochemical markers